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In brief, the sample undergoes protein denaturation, followed by gel electrophoresis.
Western blot test. A Western blot test is typically used to confirm a positive HIV diagnosis. These tests are used to detect specific proteins in a sample. The western blot, or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract.
The basic technique of a Western blot involves sorting proteins by length on a gel. Western blotting (protein blotting or immunoblotting) is a rapid and sensitive assay for detection and characterization of proteins. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed.
A synthetic or animal-derived antibody is created that recognises and binds to a specific target protein. The Western blot test, also called immunoblotting, is a test for a specific protein within a protein mixture. However, the Western blot is no longer used, and today.
The Western blot test separates the blood proteins and detects the specific proteins (called HIV antibodies) that indicate an HIV infection. Then that grid is probed with antibodies that react to the specific proteins that are being searched for. During the test, a small sample of blood is taken and it is used to detect HIV antibodies, not the HIV virus itself.
Western blot can be performed via venipuncture or by spinal tap (lumbar puncture) to test the cerebrospinal fluid. Results from a Western blot test are usually reported in terms of titers which is a measure of the amount of dilution a sample needs before no antibodies remain detectable. Western blots tests are also known as protein immunoblots.
A high titer means, therefore, a high level of antibodies and is likely to indicate a major response by the body to a current or recent infection.
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