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The reaction setup is complete with one pipetting step, which is followed by two incubations.
Exo sap. The first incubation digests excess primer and dephosphorylates nucleotides. The exonuclease I removes leftover primers, while the Shrimp Alkaline Phosphatase removes any remaining dNTPs. It is used to remove excess primers and dNTPs from a PCR mixture with no interference in downstream applications.
ExoSAP-IT Express reagent consists of two hydrolytic enzymes, a modified Exonuclease I and Shrimp Alkaline Phosphatase (SAP), in a specially formulated buffer. The ExoSAP-IT one-step solution provides a simple way to enzymatically purify a PCR reaction. ExoSAP-IT single-step PCR cleanup utilizes two hydrolytic enzymes, Exonuclease I and Shrimp Alkaline Phosphatase (SAP), together in a specially formulated buffer, to remove unwanted dNTPs and primers from PCR products.
Our sequencing Exo-SAP-IT PCR product cleanup reagent provides fast, easy, and convenient solutions for your PCR cleanup needs. ExoSAP-IT PCR Product Cleanup Reagent High-quality, accurate results, faster turnaround time It is important to achieve a clean template for your Sanger sequencing reaction without losing precious samples. The ExoSAP protocol is the simplest way to clean-up PCR products before sequencing.
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